Description
Introduction: The triggering receptor expressed on myeloid cells (TREM-1) is a recently identified activating receptor on neutrophil granulocytes (PMN), monocytes, and macrophage subsets. The expression of TREM-1 is upregulated by microbial products, that is, by toll-like receptor ligands such as lipoteichoic acid (LTA) of Gram-positive or lipopolysaccharide (LPS) of Gram-negative bacteria. Ligation of TREM-1 is synergistic with TLR agonists on the activation of receptor bearing cells for the release of inflammatory mediators like TNF-alpha and IL-8 and the initiation of neutrophil respiratory burst.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TREM-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TREM-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TREM-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm } 2 nm. The concentration of TREM-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve